Papers Describe Database and Non-animal Approach to Identifying Androgen-active Chemicals
Two recent papers in Reproductive Toxicology describe data and methods used by U.S. Environmental Protection Agency, Organisation for Economic Co-operation and Development, and NICEATM scientists and collaborators to develop a reference database and evaluate an in vitro model to identify chemicals with the potential to interact with the androgen receptor (AR). The AR model is potentially a rapid, cost-effective replacement for the in vivo Hershberger assay.
- Browne et al. describes assembly and curation of a data set of results for the Hershberger and other in vivo assays for AR activity. Ultimately, 49 chemicals were identified with reproducible AR pathway responses confirmed in at least two in vivo rodent studies that could be considered reference chemicals useful for validating alternative methods.
- Kleinstreuer et al. describes use of the reference chemicals identified through the data curation effort to interrogate the performance of a ToxCast/Tox21 AR model based on 11 high-throughput assays. The AR model had 100% positive predictive value for the in vivo response, where chemicals with conclusive AR model results were consistently positive in vivo.