New Method for Cell Line Authentication
Each type of cell line has the potential to change over time as the cells replicate in culture, or to become contaminated with another cell type or an infectious agent. All of these potential “changes” can alter the results obtained when using the cells in experiments or tests.
Several organizations, including the American Type Culture Collection and some of the organizations associated with toxicity test method validation, have developed and promote the use of good cell culture practices that include the need to characterize and identify cell lines.
A new method for cell line authentication was recently published in the open-access journal BMC Genomics. “NIAID researchers and their colleagues have described a new method to assess misidentification, contamination, and genetic abnormalities in mouse cell lines, helping to ensure the validity of research findings.”
Summary of the research paper: http://www.niaid.nih.gov/LabsAndResources/resources/bioinformatics/Pages/newCellLineAuthentication.aspx
The open-access article: http://www.biomedcentral.com/1471-2164/15/847
Didion, J.P., Buus, R.J., Naghashfar, Z., Threadgill, D.W., Morse, H.C., de Villena F.P. (2014). SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy. BMC Genomics 15: 847.
Posted: October 23, 2014