CRACK IT Solutions: Partners sought to help develop and validate a portable bioluminescence test for botulinum neurotoxin
Author: Sherry Ward, AltTox
“Researchers from University of Freiburg, Germany, are seeking partners to validate and commercialize their novel automated bioluminescence assay (BLBTest) for detecting botulinum neurotoxin (BoNT) to replace mouse bioassays.
What could the Solution be used for?
The BLB-Test is a portable, highly sensitive, automated assay for detecting BoNT as a replacement for the mouse lethality bioassay (MLB). The assay can detect and distinguish all of the disease-causing BoNT serotypes, making the BLBTest useful for analysis of samples from human and animal, foodstuff and environmental samples. The BLB-Test detects the endopeptidase activity of the light chain domain of BoNT, thereby detecting only active toxin and avoiding potential false positives caused by inactive BoNT, and can detect toxins in complex matrices without the need for sample pre-treatment.”
Need for collaboration
We seek partnerships with: ƒ End-users interested in helping to validate the BLB-Test on a broad range of complex matrices that could be contaminated with BoNT, including patient specimens, environmental samples, food and beverages, animal feed, gardening products etc. ƒ Companies interested in producing and commercializing the BLB-Test. ƒ Collaborators with expertise in the biochemistry of cellular membranes and receptors.
3Rs impact assessment
The MLB is used extensively for testing products and samples that are suspected to contain BoNT, requiring up to 20 mice per sample (i.e. >140 animals to fully serotype each specimen). It was estimated that approximately 600,000 mice were used worldwide in 2009 for diagnostics and pharmaceutical purposes. The test method causes severe distress to the animals and death by slow asphyxiation over a period of up to four days. In its present form, the BLB-Test can be used to replace the MLB in most circumstances because it can detect, distinguish and quantify all the serotypes (BoNT A-F) that are known to cause disease in humans and animals.”
For further information on this collaboration opportunity see: http://www.crackit.org.uk/news/new-solution-portable-bioluminescence-test-botulinum-neurotoxin and http://www.crackit.org.uk/portable-bioluminescence-test-botulinum-neurotoxin