Sources for in vitro protocols and summary reviews of test methods, as well as and other methods used infor non-animal toxicology research and testing.

• Non-animal Toxicity Test Protocols, Test Guidelines, Summary Reviews
• (Q)SAR/In Silico Tools
• General Laboratory Protocols

(For search strategies, search engines, and databases that can assist in locating alternative test methods, see this page: Searching for Non-animal Toxicity Test Methods)

Non-animal Toxicity Testing Protocols/Test Guidelines/Summary Reviews

3D Cell Culture Knowledge Center: 3D cell culture protocols, white papers, videos, and more

3R Research Foundation 3R Info Bulletins

AnimAlt-ZEBET: full-text database of evaluated alternative methods to animal experiments

Assay Guidance Manual: open access eBook providing guidance for developing and validating high-throughput screening (HTS) assays

CPSC’s Policy on Animal Testing: supplement to CPSC’s proposed statement of policy on animal testing to help industry determine what tests currently are acceptable and available for meeting the labeling requirements of the Federal Hazardous Substances Act (FHSA)

Current Protocols in Toxicology: subscription-based database of protocols

DB-ALM ECVAM Database Service on Alternative Methods to Animal Experimentation: includes INVITTOX database protocols and validated ECVAM test methods

EDQM Alternatives to Animal Testing webpage: 3Rs approaches in the develoment and quality control of biologicals and vaccines; animal welfare progress in European Pharmacopoeia monographs

EUROECOTOX database of bioassays

EURL ECVAM: validated ECVAM test methods and related documents

ICCVAM: validated ICCVAM test methods and related documents; U.S. and International Acceptance of Alternative Methods, 1998-2012: includes links to validated protocols and test guidelines; ICCVAM-recommended protocols

JaCVAM: JaCVAM statements on reviewed test methods

National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs): access to articles and guidelines

OECD Guidelines for the Testing of Chemicals: Test Guidelines; draft Test Guidelines; Guidance Documents; draft Guidance Documents

 (Q)SAR/In Silico Tools

JRC QSAR Model Inventory: online database which hosts structured and peer-reviewed information on QSAR Models

OECD QSAR Toolbox: software to fill gaps in (eco-)toxicity data needed for assessing the hazards of chemicals

US EPA ECOSAR (Ecological Structure Activity Relationships)

US EPA Toxicity Estimation Software Tool (T.E.S.T.): software enables toxicity estimates from a chemical’s molecular structure using (Q)SAR methodologies

More software/QSAR tools on this page: Testing Supplies, Cells, Tissues, Software

 General Laboratory Protocols

ATCC® Primary Cell Culture Guide

ATCC Stem Cell Culture Guide

BioProbes® Journal

BioTechniques Protocols Guide: annual collections of optimized procedures and protocols

Cold Spring Harbor Protocols: subscription-based journal; free trial subscription offered

Common Laboratory Recipes Calculator

Current Protocols in Cell Biology: subscription-based database of protocols

Current Protocols videos

ECACC Cell Culture Laboratory Handbook

GenomeWeb Tech Guides

Gibco® Cell Culture Basics

Immunogenicity Knowledge Base

International Stem Cell Registry Protocols

Journal of Visualized Experiments (JoVE): peer-reviewed journal publishing biological research and protocols in a video format

Life Technologies™ Protocols: cell culture, stem cell research, ADME/Tox, and more

Methods of Pharmacology and Toxicology: book series providing methods and protocols in all areas of pharmacological and toxicological research

Nature Protocols: subscription-based journal; some open-access articles

Nature Protocol Exchange: open resource where scientists can share, search for, and/or comment on a protocol

Novus Biologicals: selection of protocols and video demonstrations

Nucleic Acids Research (NAR) Methods: methods published in NAR 1999-present

Protocol Online: an online resource for laboratory protocols

Reinnervate 3D cell culture protocols

Sigma-Aldrich Applications Center

Springer Protocols: subscription-based database of reproducible laboratory protocols in the life and biomedical sciences

Stembook Protocols: stem cell protocols

Stemgent Video Library: lab protocols for stem cells